Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 447986
Title Effect of oxygen concentration and selected protocol factors on viability and gene expression of mouse liver slices
Author(s) Szalowska, E.; Stoopen, G.M.; Rijk, J.C.W.; Wang, S.; Hendriksen, P.J.M.; Groot, M.J.; Ossenkoppele, J.S.; Peijnenburg, A.A.C.M.
Source Toxicology in Vitro 27 (2013)5. - ISSN 0887-2333 - p. 1513 - 1524.
DOI https://doi.org/10.1016/j.tiv.2013.03.007
Department(s) RIKILT - BU Toxicology Bioassays & Novel Foods
Rikilt B&T Toxicologie en Effectanalyse
Sub-department of Toxicology
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) precision-cut liver - toxicity - metabolism - culture - glucose - tension - systems - cells - toxicogenomics - activation
Abstract Precision cut liver slices (PCLSs) are widely used as a model to study hepatotoxicity. For culturing of PCLS diverse protocols are used which could affect slices viability and results. We aimed to identify the most optimal culture protocol for mouse PCLS. Slices were cultured for 24 h under different concentrations of serum, glucose, insulin, and oxygen. Thereafter, slices viability was assessed by biochemical methods. Transcriptome analysis was performed to identify changes introduced by culture at different oxygen concentrations (20%, 40%, 60%, and 80% of oxygen). Medium composition did not affect the slices viability. Although metabolic competence was unaffected by oxygen concentrations, culturing at 80% of oxygen yielded slices with the best biochemical characteristics. The comparison of uncultured vs. cultured slices revealed 2524 genes to be differentially expressed. Genes involved in drug metabolism, peroxisomal and mitochondrial functions were down-regulated while several adaptive/stress response processes were up-regulated. Moreover, 80% of oxygen was the most favorable condition with respect to maintenance of expression of genes involved in drug and energy metabolism. The outcome of this study indicates that mouse PCLS are a valuable tool in research on hepatic functions and toxicity, particularly if they are cultured under a controlled oxygen concentration of 80%.
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