Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 449630
Title Laxative treatment with polyethylene glycol decreases microbial primary bile salt dehydroxylation and lipid metabolism in the intestine of rats
Author(s) Wulp, N.Y. van der; Derrien, M.; Stellaard, F.; Wolters, H.; Kleerebezem, M.; Dekker, J.; Rings, E.H.; Groen, A.K.; Verkade, H.J.
Source American Journal of Physiology. Gastrointestinal and Liver Physiology 305 (2013)7. - ISSN 0193-1857 - p. G474 - G482.
DOI http://dx.doi.org/10.1152/ajpgi.00375.2012
Department(s) Host Microbe Interactomics
WIAS
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) deoxycholic-acid formation - fecal water - enterohepatic circulation - gastrointestinal cancer - cholesterol saturation - chronic constipation - induced increase - colon-cancer - transit - bacteria
Abstract Polyethylene glycol (PEG) is a frequently used osmotic laxative that accelerates gastrointestinal transit. It has remained unclear, however, whether PEG affects intestinal functions. We aimed to determine the effect of PEG treatment on intestinal sterol metabolism. Rats were treated with PEG in drinking water (7%) for 2 wk or left untreated (controls). We studied the enterohepatic circulation of the major bile salt (BS) cholate with a plasma stable isotope dilution technique and determined BS profiles and concentrations in bile, intestinal lumen contents, and feces. We determined the fecal excretion of cholesterol plus its intestinally formed metabolites. Finally, we determined the cytolytic activity of fecal water (a surrogate marker of colorectal cancer risk) and the amount and composition of fecal microbiota. Compared with control rats, PEG treatment increased the pool size (+51%; P <0.01) and decreased the fractional turnover of cholate (-32%; P <0.01). PEG did not affect the cholate synthesis rate, corresponding with an unaffected fecal primary BS excretion. PEG reduced fecal excretion of secondary BS and of cholesterol metabolites (each P <0.01). PEG decreased the cytolytic activity of fecal water [54 (46–62) vs. 87 (85–92)% erythrocyte potassium release in PEG-treated and control rats, respectively; P <0.01]. PEG treatment increased the contribution of Verrucomicrobia (P <0.01) and decreased that of Firmicutes (P <0.01) in fecal flora. We concluded that PEG treatment changes the intestinal bacterial composition, decreases the bacterial dehydroxylation of primary BS and the metabolism of cholesterol, and increases the pool size of the primary BS cholate in rats
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