Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 449752
Title The Histone Deacetylase Inhibitor Trichostatin A Promotes Totipotency in the Male Gametophyte
Author(s) Li, H.; Soriano, M.; Cordewener, J.H.G.; Muino Acuna, J.M.; Riksen-Bruinsma, T.; Fukuoka, H.; Angenent, G.C.; Boutilier, K.A.
Source The Plant Cell 26 (2014)1. - ISSN 1040-4651 - p. 195 - 209.
DOI http://dx.doi.org/10.1105/tpc.113.116491
Department(s) PRI BIOS Applied Genomics & Proteomics
PRI BIOS Applied Bioinformatics
PRI BIOS Plant Development Systems
Laboratory of Molecular Biology
EPS-1
Publication type Refereed Article in a scientific journal
Publication year 2014
Keyword(s) brassica-napus l - polycomb-group proteins - plant-cell cycle - somatic embryogenesis - arabidopsis-thaliana - embryo development - retinoblastoma protein - in-vitro - microspore embryogenesis - pollen embryogenesis
Abstract The haploid male gametophyte, the pollen grain, is a terminally differentiated structure whose function ends at fertilization. Plant breeding and propagation widely use haploid embryo production from in vitro–cultured male gametophytes, but this technique remains poorly understood at the mechanistic level. Here, we show that histone deacetylases (HDACs) regulate the switch to haploid embryogenesis. Blocking HDAC activity with trichostatin A (TSA) in cultured male gametophytes of Brassica napus leads to a large increase in the proportion of cells that switch from pollen to embryogenic growth. Embryogenic growth is enhanced by, but not dependent on, the high-temperature stress that is normally used to induce haploid embryogenesis in B. napus. The male gametophyte of Arabidopsis thaliana, which is recalcitrant to haploid embryo development in culture, also forms embryogenic cell clusters after TSA treatment. Genetic analysis suggests that the HDAC protein HDA17 plays a role in this process. TSA treatment of male gametophytes is associated with the hyperacetylation of histones H3 and H4. We propose that the totipotency of the male gametophyte is kept in check by an HDAC-dependent mechanism and that the stress treatments used to induce haploid embryo development in culture impinge on this HDAC-dependent pathway.
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