Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 451585
Title Geostatistical analysis of nematode taxa on hectare scale provide essential knowledge to monitor the ecological impact of bio-based crops and residues
Author(s) Quist, C.W.; Mooijman, P.J.W.; Elsen, S.J.J. van den; Brus, D.J.; Mulder, C.; Termoshuizen, A.; Bakker, J.; Helder, J.
Event Be-Basic Symposium 2014, 2014-02-04/2014-02-06
Department(s) Laboratory of Nematology
PRI BIOS Applied Bioinformatics
Alterra - Soil geography
PE&RC
Publication type Poster (professional)
Publication year 2014
Abstract Because of their abundance, their trophic diversity and their relatively straightforward extractability, nematodes have a potential as a proxy for the biological condition of soils and sediments. So far the potential of this group is underexploited for technical reasons; the microscopic analysis of nematode assemblages is labour intensive and requires extensive expertise. With the availability of a relatively large molecular framework, it became possible to design molecular assays that allow for the quantitative analysis of individual taxa against complex DNA backgrounds (Vervoort et al. 2012). The availability of high throughput tools to monitor nematode assemblages makes it possible to investigate the spatial distribution of nematode taxa at field level. In order to assess the degree of patchiness of individual nematode taxa (and this relates directly to the strategy that should be used to sample them), 12 fields (4 from each marine clay, river clay and sandy soils) of a single hectare each were investigated in great detail. Using a sampling grid optimized for geostatistic analysis, 96 composite samples were taken from each of these fields. Each of these samples were analysed with 24 molecular assays (corresponding to 23 nematode taxa and one internal control). In total over 30,000 qPCR reactions were run, a preliminary results (visualized as semi-variograms and surface maps) show a great variation in distribution patterns not only on the basis of nematode taxa but also based on soil type. The data that will be presented are essential for the design of scientifically sound sampling schemes to monitor the ecological impact of bio-based crops and residues on arable field scale.
Comments
There are no comments yet. You can post the first one!
Post a comment
 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.