Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 455988
Title Critical parameters in cost-effective alkaline extraction for high protein yield from leaves
Author(s) Zhang, C.; Sanders, J.P.M.; Bruins, M.E.
Source Biomass and Bioenergy 67 (2014). - ISSN 0961-9534 - p. 466 - 472.
DOI https://doi.org/10.1016/j.biombioe.2014.05.020
Department(s) Biobased Chemistry and Technology
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2014
Keyword(s) leaf protein - functional-properties - chemical-composition - amino-acids - tea-leaves - biomass - concentrate - refinery - feed
Abstract Leaves are potential resources for feed or food, but their applications are limited due to a high proportion of insoluble protein and inefficient processing. To overcome these problems, parameters of alkaline extraction were evaluated using green tea residue (GTR). Protein extraction could be maximized to 95% of total protein, and, after precipitation by pH adjustment to 3.5, 85% of extracted protein was recovered with a purity of 52%. Temperature, NaOH amount, and extraction time are the protein yield determining parameters, while pH and volume of extraction liquid are critical parameters for production cost. The cost of energy and chemicals for producing 1 t GTR proteins is minimized to 102€, and its nutritional value is comparable to soybean protein. Furthermore, this technology was successfully applied to other sources of biomass and has potential to be used as a part of an integrated bio-refinery process.
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