Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 456149
Title Miscellaneous standard methods for Apis mellifera research
Author(s) Human, H.; Brodschneider, R.; Dietemann, V.; Dively, G.; Ellis, J.D.; Forsgren, E.; Fries, I.; Hatjina, F.; Hu, F.L.; Jaffe, R.; Jensen, A.B.; Kohler, A.; Magyar, J.P.; Ouml;zkyrym, A.; Pirk, C.W.W.; Rose, R.; Strauss, U.; Tanner, G.; Tarpy, D.R.; Steen, J.J.M. van der; Vaudo, A.; Vejsnaes, F.; Wilde, J. de; Williams, G.R.; Zheng, H.Q.
Source Journal of Apicultural Research 52 (2013)4. - ISSN 0021-8839
DOI http://dx.doi.org/10.3896/IBRA.1.52.4.10
Department(s) Sub-department of Environmental Technology
PRI Bioint Entomology & Disease Management
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) honey-bee workers - greatheadii var. davyana - low-temperature narcosis - varroa-jacobsoni oud - carbon-dioxide - nosema-ceranae - developmental stages - nutritional content - drone congregation - natural conditions
Abstract A variety of methods are used in honey bee research and differ depending on the level at which the research is conducted. On an individual level, the handling of individual honey bees, including the queen, larvae and pupae are required. There are different methods for the immobilising, killing and storing as well as determining individual weight of bees. The precise timing of developmental stages is also an important aspect of sampling individuals for experiments. In order to investigate and manipulate functional processes in honey bees, e. g. memory formation and retrieval and gene expression, microinjection is often used. A method that is used by both researchers and beekeepers is the marking of queens that serves not only to help to locate her during her life, but also enables the dating of queens. Creating multiple queen colonies allows the beekeeper to maintain spare queens, increase brood production or ask questions related to reproduction. On colony level, very useful techniques are the measurement of intra hive mortality using dead bee traps, weighing of full hives, collecting pollen and nectar, and digital monitoring of brood development via location recognition. At the population level, estimation of population density is essential to evaluate the health status and using beelines help to locate wild colonies. These methods, described in this paper, are especially valuable when investigating the effects of pesticide applications, environmental pollution and diseases on colony survival.
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