Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 479773
Title Circadian rhythms in the cell cycle and biomass composition of Neochloris oleoabundans under nitrogen limitation
Author(s) Winter, L. de; Schepers, L.W.; Cuaresma Franco, M.; Barbosa, M.J.; Martens, D.E.; Wijffels, R.H.
Source Journal of Biotechnology 187 (2014). - ISSN 0168-1656 - p. 25 - 33.
DOI https://doi.org/10.1016/j.jbiotec.2014.07.016
Department(s) Bioprocess Engineering
FBR Bioconversion
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2014
Keyword(s) chlamydomonas-reinhardtii - nutrient starvation - microalgae - division - growth - accumulation - quadricauda - clock - death
Abstract The circadian clock schedules processes in microalgae cells at suitable times in the day/night cycle. To gain knowledge about these biological time schedules, Neochloris oleoabundans was grown under constant light conditions and nitrogen limitation. Under these constant conditions, the only variable was the circadian clock. The results were compared to previous work done under nitrogen-replete conditions, in order to determine the effect of N-limitation on circadian rhythms in the cell cycle and biomass composition of N. oleoabundans. The circadian clock was not affected by nitrogen-limitation, and cell division was timed in the natural night, despite of constant light conditions. However, because of nitrogen-limitation, not the entire population was able to divide every day. Two subpopulations were observed, which divided alternately every other day. This caused oscillations in biomass yield and composition. Starch and total fatty acids (TFA) were accumulated during the day. Also, fatty acid composition changed during the cell cycle. Neutral lipids were built up during the day, especially in cells that were arrested in their cell cycle (G2 and G3). These findings give insight in the influence of circadian rhythms on the cell cycle and biomass composition.
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