Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 494729
Title A simple routine for quantitative analysis of light and dark kinetics of photochemical and non-photochemical quenching of chlorophyll fluorescence in intact leaves
Author(s) Vredenberg, Wim
Source Photosynthesis Research 124 (2015)1. - ISSN 0166-8595 - p. 87 - 106.
Department(s) Laboratory of Plant Physiology
Publication type Refereed Article in a scientific journal
Publication year 2015
Keyword(s) Chlorophyll fluorescence kinetics - OJIP - Quenching mechanisms - System analysis - TSTM - Views

Paper describes principles and application of a novel routine that enables the quantitative analysis of the photochemical O-J phase of the variable fluorescence F v associated with the reversible photo-reduction of the secondary electron acceptor QA of photosystem II (PSII) in algae and intact leaves. The kinetic parameters that determine the variable fluorescence F PP(t) associated with the release of photochemical quenching are estimated from 10 μs time-resolved light-on and light-off responses of F v induced by two subsequent light pulses of 0.25 (default) and 1000 ms duration, respectively. Application of these pulses allows estimations of (i) the actual value of the rate constants k L and k AB of the light excitation (photoreduction of QA) and of the dark re-oxidation of photoreduced QA (QA -), respectively, (ii) the actual maximal normalized variable fluorescence [nF v] associated with 100 % photoreduction of QA of open RCs, and (iii) the actual size β of RCs in which the re-oxidation of QA - is largely suppressed (QB-nonreducing RC with k AB ~ 0). The rate constants of the dark reversion of Fv associated with the release of photo-electrochemical quenching F PE and photo-electric stimulation F CET in the successive J-I and I-P parts of the thermal phase are in the range of (100 ms)-1 and (1 s)-1, respectively. The kinetics of fluorescence changes during and after the I-P phase are given special attention in relation to the hypothesis on the involvement of a Δμ H+-dependent effect during this phase and thereafter. Paper closes with author's personal view on the demands that should be fulfilled for chlorophyll fluorescence methods being a correct and unchallenged signature of photosynthesis in algae and plants.

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