Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 494734
Title THP-1 and U937 cells
Author(s) Chanput, Wasaporn; Peters, Vera; Wichers, Harry
Source In: The Impact of Food Bioactives on Health: In Vitro and Ex Vivo Models Springer International Publishing - ISBN 9783319161044 - p. 147 - 159.
DOI http://dx.doi.org/10.1007/978-3-319-16104-4_14
Department(s) Chair Nutrition and Health over the Lifecourse
Laboratory of Molecular Biology
FBR Consumer Science & Health
VLAG
Publication type Peer reviewed book chapter
Publication year 2015
Keyword(s) Cell line - Macrophage - Monocyte - THP-1 - U937
Abstract

Monocytes are circulatory precursor cells from myeloid origin that can develop into macrophages or dendritic cells upon migration from the blood stream to tissues. Both macrophages and dendritic cells are professional antigen-presenting cells. Monocytes and their macrophage and dendritic-cell progeny serve three main functions in the immune system. These are phagocytosis, antigen presentation, and cytokine production. THP-1 and U937 are (pro-) monocytic cell lines that can, also in vitro, be differentiated into either various types of macrophages or into dendritic cells. This chapter describes how to grow THP-1, resp. U937 cells, how to differentiate these into more specialised phenotypes such as various macrophage types or dendritic cells, how to read-out their responses to stimuli and it gives examples of how such cell lines have been used into studying the effects of food compounds.

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