|Title||Endo-glucanase digestion of oat β-Glucan enhances Dectin-1 activation in human dendritic cells|
|Author(s)||Sahasrabudhe, N.M.; Tian, Lingmin; Berg, Marco van den; Bruggeman, Geert; Bruininx, Erik; Schols, H.A.; Faas, M.M.; Vos, Paul de|
|Source||Journal of Functional Foods 21 (2016). - ISSN 1756-4646 - p. 104 - 112.|
Food Chemistry Group
|Publication type||Refereed Article in a scientific journal|
|Keyword(s)||Dectin-1 - Dendritic cells - Endo-glucanase - Oat - Particle size - β-(1-3, 1-4) Glucan|
Oat β-Glucans were studied for their immunological impact before and after enzymatic digestion in order to enhance the efficacy of oat β-Glucans for application in functional foods. Oat β-Glucan is reported to have minimal impact compared to its fungal counterpart in vitro. Digestion with endo-glucanase enhanced its efficacy towards stimulating MCP-1, RANTES, IL-8, and IL-production in human dendritic cells as compared to the nondigested β-Glucan. This effect resulted from an enhanced activation of the Dectin-receptor. Our data suggest that the immune-stimulation was dependent on the β-(1-3) linkages and the reduced particle size of digested β-Glucans. Thus, we show that enzymatic pre-digestion of dietary fibres such as oat β-Glucan enhances its impact on specific immune receptors. We also demonstrate that particle size and/or molecular weight of oat β-Glucans and exposure of specific binding sites for the receptors might be important tools for designing efficacious functional feed and food additives.