Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 494781
Title Endo-glucanase digestion of oat β-Glucan enhances Dectin-1 activation in human dendritic cells
Author(s) Sahasrabudhe, N.M.; Tian, Lingmin; Berg, Marco van den; Bruggeman, Geert; Bruininx, Erik; Schols, H.A.; Faas, M.M.; Vos, Paul de
Source Journal of Functional Foods 21 (2016). - ISSN 1756-4646 - p. 104 - 112.
DOI http://dx.doi.org/10.1016/j.jff.2015.11.037
Department(s) Food Chemistry Group
Animal Nutrition
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) Dectin-1 - Dendritic cells - Endo-glucanase - Oat - Particle size - β-(1-3, 1-4) Glucan
Abstract

Oat β-Glucans were studied for their immunological impact before and after enzymatic digestion in order to enhance the efficacy of oat β-Glucans for application in functional foods. Oat β-Glucan is reported to have minimal impact compared to its fungal counterpart in vitro. Digestion with endo-glucanase enhanced its efficacy towards stimulating MCP-1, RANTES, IL-8, and IL-production in human dendritic cells as compared to the nondigested β-Glucan. This effect resulted from an enhanced activation of the Dectin-receptor. Our data suggest that the immune-stimulation was dependent on the β-(1-3) linkages and the reduced particle size of digested β-Glucans. Thus, we show that enzymatic pre-digestion of dietary fibres such as oat β-Glucan enhances its impact on specific immune receptors. We also demonstrate that particle size and/or molecular weight of oat β-Glucans and exposure of specific binding sites for the receptors might be important tools for designing efficacious functional feed and food additives.

Comments
There are no comments yet. You can post the first one!
Post a comment
 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.