Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 494810
Title Hepatic genome-wide expression of lipid metabolism in diet-induced obesity rats treated with cocoa polyphenols
Author(s) Ali, Faisal; Ismail, Amin; Esa, Norhaizan Mohd; Pei, Chong Pei; Kersten, Sander
Source Journal of Functional Foods 17 (2015). - ISSN 1756-4646 - p. 969 - 978.
DOI http://dx.doi.org/10.1016/j.jff.2015.06.047
Department(s) Chair Nutrition Metabolism and Genomics
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2015
Keyword(s) Cocoa polyphenols treatment - DNA microarray - Genes expression profiling - Lipid metabolism - Liver steatosis - Obesity
Abstract

Cocoa polyphenols (CPs) have been shown to exhibit hypolipidaemic actions, suggesting that CPs offer great potential for ameliorating lipid abnormalities. However, the conceivable molecular mechanisms underlying the pharmacological activity of CPs in obesity-induced liver steatosis have yet to be an investigated. This study analysed the hepatic genome-wide expression patterns in high-fat diet (HFD)-induced obese rats using DNA microarray. Rats were fed either a low fat (LFD) or high fat diet (HFD) for 12 weeks. After supplementation, HFD rats (n = 10/group) were treated with 600 mg/kg bw/day CPs (HFD + CPs) for 4 weeks. As a result, compared to the HFD group, CP treatment significantly lowered lipid in the liver and attenuated the increases in body weight as well as visceral fat accumulation in the CP group. DNA microarray analysis resulted in a differential expression of 862 genes of the 12,282 genes expressed in the liver. The differential expression patterns of selected genes were validated with real-time-PCR. Metabolic pathway analysis via bioinformatic tools showed that genes in lipid catabolism, primarily in fatty acid oxidation, were up-regulated in the CP group, whereas genes in lipid synthesis pathways were down-regulated. Together, these findings provide a novel insight into possible molecular mechanisms behind the pharmacological actions of CPs on the management of the obesity-induced steatosis markers in rats with diet-induced obesity.

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