Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 495088
Title The Rx1_'Gpa2 locus in potato: a molecular and genetic framework for engineering novel NLR genes
Author(s) Slootweg, E.J.; Roosien, J.; Bakker, E.H.; Pomp, H.; Bakker, J.; Goverse, A.
Event 3rd Annual Conference of the COST Action Sustain (FA1208), Banyuls s/ Mer, 2016-02-17/2016-02-19
Department(s) Laboratory of Nematology
Publication type Abstract in scientific journal or proceedings
Publication year 2016
Abstract Plants are constantly exposed to a diverse array of pathogens and parasites that attempt to invade leafs, stems, or roots by various mechanisms. To sense foreign invaders, plants have evolved a cell autonomous immune system consisting of specific receptor-like proteins, including nucleotide binding domain and leucine-rich repeat containing proteins (NLRs), which confer host specific resistance upon recognition of pathogen elicitors. The close homologs Gpa2 and Rx1 confer resistance in potato to taxonomically unrelated pathogens: the cyst nematode Globodera pallida and Potato virus X (PVX), respectively. This provides us with a model system to study evolutionary and molecular aspects involved in pathogen recognition and NLR activation in plants. Our results demonstrate that complex NLR loci provide a genetic framework in which intergenic sequence exchange between homologous genes is allowed, but also point to functional constraints that act on the generation of effective novel NLR proteins. Sequence exchange results often in gain or loss of function phenotypes due to incompatibility between functional domains involved in regulating the molecular switch function of these proteins. However, functionality can be restored by modulating the sensitivity of the protein. This knowledge contributes to a better understanding of NLR evolution, but provides us also with a functional framework for engineering novel NLR genes based on gene shuffling and targeted mutagenesis.
There are no comments yet. You can post the first one!
Post a comment
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.