Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 495854
Title Tomato spotted wilt virus in Sri Lanka: Emerging problems of Tospovirues
Author(s) Widana Gamage, S.M.K.; Hassani Mehraban, A.; Peters, D.
Source Tropical Agricultural Research & Extension 16 (2015)3. - ISSN 1391-3646 - p. 66 - 73.
DOI http://dx.doi.org/10.4038/tare.v16i3.5278
Department(s) Laboratory of Virology
Publication type Refereed Article in a scientific journal
Publication year 2015
Abstract Tomato spotted wilt virus (TSWV), the type species of the genus Tospovirus has long been reported infecting economically important horticultural crops such as tomato, groundnut, pepper, potato and soybean, all over the world. In Sri Lanka, characteristic tospovirus symptoms such as bud necrosis, axillary shoot proliferation and ring spots were observed on leaves of groundnut in Angunakolapalassa, and concentric rings on leaves and fruits of tomato, in Ambalantota and Gannoruwa. Sap extracted from both tomato and groundnut plants was serologically positive for TSWV infection in a double antibody sandwich enzyme-linked immunosorbentassay (DASELISA), but not for Groundnut bud necrosis virus (GBNV). Nucleocapsid (N) genes of both isolates were amplified in Reverse Transcription Polymerase Chain Reactions (RT-PCR) using primers specific for TSWV and GBNV. The sequences obtained showed 98% amino acid sequence identity to the N gene of the Brazilian isolate of TSWV-BR-01. This study confirms the presence of TSWV infecting groundnut and tomato in Sri Lanka.
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