Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 495889
Title Modulation of (-)-epicatechin metabolism by coadministration with other polyphenols in caco-2 cell model
Author(s) Sanchez-Bridge, Belén; Lévèques, Antoine; Li, Hequn; Bertschy, Emmanuelle; Patin, Amaury; Actis-Goretta, Lucas
Source Drug Metabolism and Disposition 43 (2015)1. - ISSN 0090-9556 - p. 9 - 16.
DOI https://doi.org/10.1124/dmd.114.060590
Department(s) Sub-department of Toxicology
Publication type Refereed Article in a scientific journal
Publication year 2015
Abstract

Widely consumed beverages such as red wine, tea, and cocoaderived products are a great source of flavanols. Epidemiologic and interventional studies suggest that cocoa flavanols such as (- )-epicatechin may reduce the risk of cardiovascular diseases. The interaction of ( - )-epicatechin with food components including other polyphenols could modify its absorption, metabolism, and finally its bioactivity. In the present study we investigate (- )-epicatechin absorption and metabolism when coexposed with other polyphenols in the intestinal absorptive Caco-2 cell model. Depending on the type of polyphenols coadministered, the total amount of 39-O-methyl-epicatechin and 3 ′- O-sulfate-epicatechin conjugates found both in apical and basal compartments ranged from 19 to 801 nM and from 6 to 432 nM, respectively. The coincubation of ( - )-epicatechin with flavanols, chlorogenic acid, and umbelliferone resulted in similar amounts of 3′-O-methyl-epicatechin effluxed into the apical compartment relative to control. Coincubation with isorhamnetin, kaempferol, diosmetin, nevadensin, chrysin, equol, genistein, and hesperitin promoted the transport of 3′-O-methyl-epicatechin toward the basolateral side and decreased the apical efflux. Quercetin and luteolin considerably inhibited the appearance of this ( - )-epicatechin conjugate both in the apical and basolateral compartments. In conclusion, we could demonstrate that the efflux of ( - )-epicatechin conjugates to the apical or basal compartments of Caco-2 cells is modulated by certain classes of polyphenols and their amount. Ingesting ( - )-epicatechin with specific polyphenols could be a strategy to increase the bioavailability of (-)-epicatechin and to modulate its metabolic profile.

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