Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 496114
Title Simultaneous determination of seven gestagens in kidney fats by Ultra Performance Convergence Chromatography tandem mass spectrometry
Author(s) Tao, Yanfei; Balzer-Rutgers, Paula; Stolker, A.A.M.; Chen, Dongmei; Yuan, Zonghui
Source Journal of Chromatography. B, Analytical technologies in the biomedical and life sciences 988 (2015). - ISSN 1570-0232 - p. 143 - 148.
DOI http://dx.doi.org/10.1016/j.jchromb.2015.02.034
Department(s) RIKILT - Business unit Dierbehandelingsmiddelen
RIKILT - Business unit Contaminants & Toxins
Publication type Refereed Article in a scientific journal
Publication year 2015
Keyword(s) Gestagens - Kidney fat - Residues analysis - Ultra-performance convergence chromatography
Abstract

An ultra-performance convergence chromatography (UPC2) system coupled tandem mass spectrometry was successfully utilised to analyse chlormadinone acetate, delmadinone acetate, fluorogestone acetate, medroxyprogesterone acetate, megestrol acetate, melengestrol acetate, chlortestasterone acetate in bovine and porcine kidney fat. This novel approach obtained an improved resolution in comparison to previously reported chromatographic methods combined with MS detector in a shorter analytical time. All the acetylgestagen compounds were well separated on a ACQUITY UPC2 HSS C18 column (3.0×100mm, 1.7μm) by applying methanol and carbon dioxide (2/98). The LOQ of delmadinone acetate, melengestrol acetate, medroxyprogesterone acetate and megestrol acetate are 0.5μg/kg, fluorogestone acetate, chlormadinone acetate and chlortestasterone acetate 1.0μg/kg. The recoveries of gestagens spiked in kidney fats at a concentration range of 0.5 to 4μg/kg were above 86.1% with relative standard deviations (RSD) less than 13.1%. These rapid and reliable methods can be used to efficiently separate, characterize and quantify the residues of gestagens in kidney fats with advantages of shorter time, more sensitive and environmental friendly.

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