|Title||Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of Xanthomonas arboricola pv. pruni in plant tissue samples|
|Author(s)||Palacio-Bielsa, Ana; López-Soriano, Pablo; Bühlmann, Andreas; Doorn, Joop van; Pham, Khanh; Cambra, Miguel A.; Berruete, Isabel M.; Pothier, Joël F.; Duffy, Brion; Olmos, Antonio; López, María M.|
|Source||Journal of Microbiological Methods 112 (2015). - ISSN 0167-7012 - p. 36 - 39.|
|Department(s)||PPO BBF Team Lisse|
|Publication type||Refereed Article in a scientific journal|
|Keyword(s)||Diagnostic assays - LAMP - Real-time PCR - Xanthomonas arboricola pv. pruni|
Operational capacity of real-time PCR and loop-mediated isothermal amplification (LAMP) diagnostic assays for detection of Xanthomonas arboricola pv. pruni was established in a ring-test involving four laboratories. Symptomatic and healthy almond leaf samples with two methods of sample preparation were analyzed. Kappa coefficient, sensitivity, specificity, likelihood ratios and post-test probability of detection were estimated to manage the risk associated with the use of the two methods.