Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 496130
Title Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of Xanthomonas arboricola pv. pruni in plant tissue samples
Author(s) Palacio-Bielsa, Ana; López-Soriano, Pablo; Bühlmann, Andreas; Doorn, Joop van; Pham, Khanh; Cambra, Miguel A.; Berruete, Isabel M.; Pothier, Joël F.; Duffy, Brion; Olmos, Antonio; López, María M.
Source Journal of Microbiological Methods 112 (2015). - ISSN 0167-7012 - p. 36 - 39.
Department(s) PPO BBF Team Lisse
Publication type Refereed Article in a scientific journal
Publication year 2015
Keyword(s) Diagnostic assays - LAMP - Real-time PCR - Xanthomonas arboricola pv. pruni

Operational capacity of real-time PCR and loop-mediated isothermal amplification (LAMP) diagnostic assays for detection of Xanthomonas arboricola pv. pruni was established in a ring-test involving four laboratories. Symptomatic and healthy almond leaf samples with two methods of sample preparation were analyzed. Kappa coefficient, sensitivity, specificity, likelihood ratios and post-test probability of detection were estimated to manage the risk associated with the use of the two methods.

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