Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 496289
Title Monolith immuno-affinity enrichment liquid chromatography tandem mass spectrometry for quantitative protein analysis of recombinant bovine somatotropin in serum
Author(s) Smits, N.G.E.; Blokland, M.H.; Wubs, K.L.; Nessen, M.A.; Ginkel, L.A. van; Nielen, M.W.F.
Source Analytical and Bioanalytical Chemistry 407 (2015)20. - ISSN 1618-2642 - p. 6041 - 6050.
DOI http://dx.doi.org/10.1007/s00216-015-8775-2
Department(s) RIKILT - Business unit Dierbehandelingsmiddelen
RIKILT Directie
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2015
Keyword(s) bST - Immuno-affinity enrichment - Liquid chromatography - Mass spectrometry - Monolith micro-column - Recombinant bovine somatotropin
Abstract

The use of recombinant bovine somatotropin (rbST) to enhance milk production is approved in several countries, but it is prohibited in the European Union. According to EU legislation, it is necessary to confirm positive screening results prior to enforcement. Although adequate screening assays are available nowadays, development of liquid chromatography tandem mass spectrometry (LC-MS/MS) confirmatory methods to detect low levels of rbST is still a challenge. Here, we present a novel approach using immuno-affinity enrichment on monolithic micro-columns in combination with state-of-the-art ultra-high pressure LC-MS/MS (UHPLC-MS/MS) detection. The developed approach enables detection and confirmation of rbST in serum at a decision limit (CCα) concentration of 0.8 ng mL−1. Furthermore, the method is easy to handle, robust and reproducible. We successfully applied the confirmatory method to serum samples from rbST treated cows that were found suspect after immunoassay-based screening. The use of rbST could be confirmed over 1 week after treatment, and the developed method demonstrated the sensitivity needed for effective control. [Figure not available: see fulltext.]

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