Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 496292
Title Humic Acid-Like and Fulvic Acid-Like Inhibition on the Hydrolysis of Cellulose and Tributyrin
Author(s) Fernandes, T.V.; Lier, J.B. van; Zeeman, Grietje
Source Bio Energy Research 8 (2015)2. - ISSN 1939-1234 - p. 821 - 831.
Department(s) Sub-department of Environmental Technology
Publication type Refereed Article in a scientific journal
Publication year 2015
Keyword(s) Anaerobic hydrolysis inhibition - Cellulose - Humic and fulvic acids - Lipids - Repolymerisation

Enzymatic hydrolysis of complex wastes is a critical step for efficient biogas production in anaerobic digesters. Inhibition of this hydrolytic step was studied by addition of humic acid-like (HAL) and fulvic acid-like (FAL) substances, extracted from maize silage and fresh cow manure, to batch tests with cellulose and cellulases from Aspergillus niger and tributyrin and lipases from Candida rugosa. To confirm the inhibition results, microbially catalysed hydrolysis trials were performed, where Fibrobacter succinogenes was grown exclusively on cellulose. Hydrolysis products, as glucose, glycerol and butyric acid, were measured to determine the hydrolysis efficiency and its rate. Cellulose hydrolysis was inhibited by 0.5 to 5.0 g l−1 of HAL and FAL substances extracted from maize and cow manure, which are relevant concentrations for manure and maize digestion. Tributyrin hydrolysis, on the other hand, was only inhibited by 0.5 to 5.0 g l−1 of HAL substances extracted from maize and cow manure and not by FAL substances.

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