Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 496306
Title Proteomic analysis of the plasma membrane-movement tubule complex of cowpea mosaic virus
Author(s) Hollander, P.W. Den; Sousa Geraldino Duarte, Priscilla de; Bloksma, Hanke; Boeren, Sjef; Lent, J.W.M. van
Source Archives of Virology (2016). - ISSN 0304-8608 - p. 1309 - 1314.
DOI http://dx.doi.org/10.1007/s00705-016-2757-3
Department(s) Laboratory of Virology
Biochemistry
EPS
Publication type Refereed Article in a scientific journal
Publication year 2016
Abstract

Cowpea mosaic virus forms tubules constructed from the movement protein (MP) in plasmodesmata (PD) to achieve cell-to-cell movement of its virions. Similar tubules, delineated by the plasma membrane (PM), are formed protruding from the surface of infected protoplasts. These PM-tubule complexes were isolated from protoplasts by immunoprecipitation and analysed for their protein content by tandem mass spectrometry to identify host proteins with affinity for the movement tubule. Seven host proteins were abundantly present in the PM-tubule complex, including molecular chaperonins and an AAA protein. Members of both protein families have been implicated in establishment of systemic infection. The potential role of these proteins in tubule-guided cell-cell transport is discussed.

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