Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 496355
Title Thermodynamic and structural properties of tuber starches from transgenic potato plants grown in vitro and in vivo
Author(s) Wasserman, L.A.; Sergeev, A.I.; Vasil'Ev, V.G.; Plashchina, I.G.; Aksenova, N.P.; Konstantinova, T.N.; Golyanovskaya, S.A.; Sergeeva, Lidia; Romanov, G.A.
Source Carbohydrate Polymers 125 (2015). - ISSN 0144-8617 - p. 214 - 223.
DOI https://doi.org/10.1016/j.carbpol.2015.01.084
Department(s) Laboratory of Plant Physiology
EPS
Publication type Refereed Article in a scientific journal
Publication year 2015
Keyword(s) AtPHYB gene - Crystalline lamellae - DSC - Solanum tuberosum - Starch - Thermodynamic parameters - Transgenic potato - Tubers rol genes
Abstract

Potato plants harboring Phytochrome B (PHYB) gene from Arabidopsis thaliana or rol genes from Agrobacterium rhizogenes were used to study the effect of transgene expression on structure and properties of starch in tubers. Thermodynamic characteristics of starch (melting temperature, enthalpy of melting, thickness of crystalline lamellae) were shown to be variable depending on the transgene expression and plant culturing mode: in vitro or in soil. The expression of rolB or rolC genes in in vitro cultured plants evoked opposite effects on starch melting temperature and crystalline lamellae thickness. AtPHYB or rolB expression in the soil-grown potato led to the formation of more defective or more ordered starch structures, respectively, in comparison with starches of the same lines grown in vitro. On the whole, our study revealed genotype-dependent differences between starches extracted from tubers of in vitro or in vivo grown plants.

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