|Title||Thermodynamic and structural properties of tuber starches from transgenic potato plants grown in vitro and in vivo|
|Author(s)||Wasserman, L.A.; Sergeev, A.I.; Vasil'Ev, V.G.; Plashchina, I.G.; Aksenova, N.P.; Konstantinova, T.N.; Golyanovskaya, S.A.; Sergeeva, Lidia; Romanov, G.A.|
|Source||Carbohydrate Polymers 125 (2015). - ISSN 0144-8617 - p. 214 - 223.|
Laboratory of Plant Physiology
|Publication type||Refereed Article in a scientific journal|
|Keyword(s)||AtPHYB gene - Crystalline lamellae - DSC - Solanum tuberosum - Starch - Thermodynamic parameters - Transgenic potato - Tubers rol genes|
Potato plants harboring Phytochrome B (PHYB) gene from Arabidopsis thaliana or rol genes from Agrobacterium rhizogenes were used to study the effect of transgene expression on structure and properties of starch in tubers. Thermodynamic characteristics of starch (melting temperature, enthalpy of melting, thickness of crystalline lamellae) were shown to be variable depending on the transgene expression and plant culturing mode: in vitro or in soil. The expression of rolB or rolC genes in in vitro cultured plants evoked opposite effects on starch melting temperature and crystalline lamellae thickness. AtPHYB or rolB expression in the soil-grown potato led to the formation of more defective or more ordered starch structures, respectively, in comparison with starches of the same lines grown in vitro. On the whole, our study revealed genotype-dependent differences between starches extracted from tubers of in vitro or in vivo grown plants.