Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 496566
Title Analysis of inheritance mode in chrysanthemum using EST-derived SSR markers
Author(s) Park, Sang Kun; Arens, Paul; Esselink, Danny; Lim, Jin Hee; Shin, Hak Ki
Source Scientia Horticulturae 192 (2015). - ISSN 0304-4238 - p. 80 - 88.
DOI http://dx.doi.org/10.1016/j.scienta.2015.05.009
Department(s) WUR PB Siergewassen, Tissue Culture
WUR PB Biodiversiteit en Genetische Variatie
Publication type Refereed Article in a scientific journal
Publication year 2015
Keyword(s) Allele dosage - Chrysanthemum x morifolium - Dendranthema x grandiflorum - Genotyping - Polyploidy - Segregation type
Abstract

To study the inheritance mode of hexaploid chrysanthemum (random or preferential chromosome pairing), a segregation analysis was carried out using SSR markers derived from chrysanthemum ESTs in the public domain. A total of 248 EST-SSR primer pairs were screened in chrysanthemum cultivars 'Dancer' and 'Puma White', of which 49 EST-SSRs were selected as polymorphic and informative markers. These polymorphic markers were used for genotyping a F1-pseudo test cross population derived from a cross between these two cultivars. The 49 EST-SSRs detected 210 marker alleles with an average number of 4.29 marker alleles per locus. For 180 of these polymorphic SSR marker alleles, segregation could be estimated using a χ2 goodness of fit test (α=0.05) with the expected segregation ratios for hexasomic or disomic inheritance. For 65 SSR marker alleles the segregation ratio was informative for the type of inheritance, 33 marker alleles gave a good fit to the expected segregation ratio for hexasomic inheritance and whereas 24 marker alleles gave a good fit for disomic inheritance showing a higher number of marker alleles supporting autopolyploid segregation in chrysanthemum. In addition, the observed ratio of non-simplex to simplex markers was 20:80 (25 vs. 99) supported hexasomic inheritance. Furthermore, random marker allele assortment was found within the six fully informative markers giving conclusive evidence for hexasomic inheritance in chrysanthemum at these chromosomal regions.

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