|Title||Monitoring microbial metabolites using an inductively coupled resonance circuit|
|Author(s)||Karnaushenko, Daniil; Baraban, Larysa; Ye, Dan; Uguz, Ilke; Mendes, Rafael G.; Rümmeli, Mark H.; Visser, Arjan de; Schmidt, Oliver G.; Cuniberti, Gianaurelio; Makarov, Denys|
|Source||Scientific Reports 5 (2015). - ISSN 2045-2322 - 13 p.|
Laboratory of Genetics
|Publication type||Refereed Article in a scientific journal|
We present a new approach to monitor microbial population dynamics in emulsion droplets via changes in metabolite composition, using an inductively coupled LC resonance circuit. The signal measured by such resonance detector provides information on the magnetic field interaction with the bacterial culture, which is complementary to the information accessible by other detection means, based on electric field interaction, i.e. capacitive or resistive, as well as optical techniques. Several charge-related factors, including pH and ammonia concentrations, were identified as possible contributors to the characteristic of resonance detector profile. The setup enables probing the ionic byproducts of microbial metabolic activity at later stages of cell growth, where conventional optical detection methods have no discriminating power.