Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 496605
Title Whole Genome PCR Scanning (WGPS) of C. burnetii strains from ruminants
Author(s) Sidi-Boumedine, Karim; Adam, Gilbert; Angen, Oysten; Aspán, A.; Bossers, A.; Roest, H.I.J.; Prigent, Myriam; Thiéry, R.; Rousset, Elodie
Source Microbes and Infection 17 (2015)11-12. - ISSN 1286-4579 - p. 772 - 775.
DOI http://dx.doi.org/10.1016/j.micinf.2015.08.003
Department(s) CVI Infection Biology
CVI Bacteriology and Epidemiology
Publication type Refereed Article in a scientific journal
Publication year 2015
Abstract Coxiella burnetii is the causative agent of Q fever, a zoonosis that spreads from ruminants to humans via the inhalation of aerosols contaminated by livestock's birth products. This study aimed to compare the genomes of strains isolated from ruminants by “Whole Genome PCR Scanning (WGPS)” in order to identify genomic differences. C. burnetii isolated from different ruminant hosts were compared to the Nine Mile reference strain using WGPS. The identified genomic regions of differences (RDs) were confirmed by sequencing. A set of 219 primers for amplification of 10 kbp segments covering the entire genome was obtained. The analyses revealed the presence of: i) conserved genomic regions, ii) genomic polymorphism including insertions and deletions and iii) amplification failures in some cases as well. WGPS, a descriptive approach, allowed the identification and localization of divergent genetic loci from various strains of C. burnetii which consisted of deletions, insertions and maybe genomic rearrangements. It also substantiates the role played by the IS1111 element in the genomic plasticity of C. burnetii. We believe that this approach could be combined with new sequencing technologies, as a selective/directed sequencing approach, particularly when repeated sequences are present in the analysed genomes
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