Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 496909
Title Effector-driven marker development and cloning of resistance genes against Phytophthora infestans in potato breeding clone SW93-1015
Author(s) Lenman, Marit; Ali, Ashfaq; Mühlenbock, Per; Carlson-Nilsson, Ulrika; Liljeroth, Erland; Champouret, Nicolas; Vleeshouwers, V.G.A.A.; Andreasson, Erik
Source Theoretical and Applied Genetics 129 (2016)1. - ISSN 0040-5752 - p. 105 - 115.
Department(s) Laboratory of Plant Breeding
PBR Biodiversiteit en Genetische Variatie
Publication type Refereed Article in a scientific journal
Publication year 2016

Key message: We show the usefulness of integrating effector screening in a breeding program and in resistance gene cloning, withPhytophthoraresistance in the Swedish potato breeding clone SW93-1015 as an example. Abstract: Phytophthora infestans is one of the most devastating plant pathogens worldwide. We have earlier found that the SW93-1015 potato breeding clone has an efficient resistance against P. infestans under field conditions in Sweden, which has an unusually high local diversity of the pathogen. This potato clone has characteristics that are different from classical R-gene-mediated resistance such as elevated levels of hydrogen peroxide (H2O2) under controlled conditions. Analysis of 76 F1 potato progenies from two individual crosses resulted in nearly 50 % resistant clones, from both crosses. This result suggests that the SW93-1015 clone has a simplex genotype for this trait. Screening with over 50 different P. infestans effectors, containing the conserved motif RXLR (for Arg, any amino acid, Leu, Arg), revealed a specific response to Avr2, which suggests that SW93-1015 might contain a functional homolog of the R2 resistance gene. We cloned eight R2 gene homologs from SW93-1015, whereof seven have not been described before and one gene encoded a protein identical to Rpi-ABPT. Expression of this gene in potato cultivar Désirée provided R2-specific resistance, whereas other homologues did not. Using RNAseq analyses we designed a new DNA marker for the R2 resistance in SW93-1015. In summary, we have demonstrated the use of effector screening in practical breeding material and revealed the key resistance mechanism for SW93-1015.

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