Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 496944
Title Cisgenic Rvi6 scab-resistant apple lines show no differences in Rvi6 transcription when compared with conventionally bred cultivars
Author(s) Chizzali, Cornelia; Gusberti, Michele; Schouten, H.J.; Gessler, Cesare; Broggini, G.A.L.
Source Planta 243 (2016)3. - ISSN 0032-0935 - p. 635 - 644.
DOI http://dx.doi.org/10.1007/s00425-015-2432-z
Department(s) WUR PB Biodiversiteit en Genetische Variatie
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) Apple scab - Cisgenic - Malus × domestica - Reference genes - Rvi6 - Venturia inaequalis
Abstract

Main conclusion: The expression of the apple scab resistance geneRvi6in different apple cultivars and lines is not modulated by biotic or abiotic factors.All commercially important apple cultivars are susceptible to Venturia inaequalis, the causal organism of apple scab. A limited number of apple cultivars were bred to express the resistance gene Vf from the wild apple genotype Malus floribunda 821. Positional cloning of the Vf locus allowed the identification of the Rvi6 (formerly HcrVf2) scab resistance gene that was subsequently used to generate cisgenic apple lines. It is important to understand and compare how this resistance gene is transcribed and modulated during infection in conventionally bred cultivars and in cisgenic lines. The aim of this work was to study the transcription pattern of Rvi6 in three classically bred apple cultivars and six lines of ‘Gala’ genetically modified to express Rvi6. Rvi6 transcription was analyzed at two time points using quantitative real-time PCR (RT-qPCR) following inoculation with V. inaequalis conidia or water. Rvi6 transcription was assessed in relation to five reference genes. β-Actin, RNAPol, and UBC were the most suited to performing RT-qPCR experiments on Malus × domestica. Inoculation with V. inaequalis conidia under conditions conducive to scab infection failed to produce any significant changes to the transcription level of Rvi6. Rvi6 expression levels were inconsistent in response to external treatments in the different apple cultivars, and transgenic, intragenic or cisgenic lines.

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