Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 497316
Title Monitoring soil bacteria with community-level physiological profiles using Biolog™ ECO-plates in the Netherlands and Europe
Author(s) Rutgers, Michiel; Wouterse, Marja; Drost, Sytske M.; Breure, Anton M.; Mulder, Christian; Stone, Dorothy; Creamer, Rachel E.; Winding, Anne; Bloem, Jaap
Source Applied Soil Ecology 97 (2016). - ISSN 0929-1393 - p. 23 - 35.
DOI http://dx.doi.org/10.1016/j.apsoil.2015.06.007
Department(s) Chair Soil Biology and Biological Soil Quality
Alterra - Animal ecology
PE&RC
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) Biolog - Indicator - Method - Microbial community - MicroResp - Soil
Abstract

Soil samples were analyzed with community-level physiological profiles (CLPP) using Biolog™ ECO-plates in the Netherlands Soil Monitoring Network (NSMN; 704 samples) and in a European-wide transect (73 samples). The selection of sites was based on a representative sample of major soil texture types, land uses and climate zones. This method for determination of CLPP was specifically designed to be applied in large and long-term monitoring activities. It encompassed a solution for the inoculum-density dependence of colour formation patterns in ECO-plates, through stepwise dilution to extinction of the bacterial inoculum. The CLPP in Dutch and European soil samples appeared to be reproducible and sensitive to land use and/or soil texture. Although the method is selective, CLPP based parameters correlated well with other microbial parameters and soil characteristics. Consistent patterns in CLPP and soil habitat characteristics are emerging, as brought about by environmental disturbances, land management and soil texture. The applicability of CLPP analysis in monitoring systems is discussed.

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