Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 497784
Title Compaction of isolated Escherichia coli nucleoids : Polymer and H-NS protein synergetics
Author(s) Wegner, Anna S.; Wintraecken, Kathelijne; Spurio, Roberto; Woldringh, Conrad L.; Vries, Renko de; Odijk, Theo
Source Journal of Structural Biology 194 (2016)1. - ISSN 1047-8477 - p. 129 - 137.
DOI http://dx.doi.org/10.1016/j.jsb.2016.02.009
Department(s) Aquatic Ecology and Water Quality Management
Physical Chemistry and Soft Matter
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) Dynamic light scattering - EMSA - Escherichia coli - H-NS protein - Nucleoid - Polyethylene glycol - Polymer physics - Supercoiling
Abstract

Escherichia coli nucleoids were compacted by the inert polymer polyethylene glycol (PEG) in the presence of the H-NS protein. The protein by itself appears to have little impact on the size of the nucleoids as determined by fluorescent microscopy. However, it has a significant impact on the nucleoidal collapse by PEG. This is quantitatively explained by assuming the H-NS protein enhances the effective diameter of the DNA helix leading to an increase in the depletion forces induced by the PEG. Ultimately, however, the free energy of the nucleoid itself turns out to be independent of the H-NS concentration. This is because the enhancement of the supercoil excluded volume is negligible. The experiments on the nucleoids are corroborated by dynamic light scattering and EMSA analyses performed on DNA plasmids in the presence of PEG and H-NS.

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