Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 498030
Title Porcine Ex Vivo intestinal segment model
Author(s) Ripken, D.; Hendriks, H.F.J.
Source In: The Impact of Food Bioactives on Health: In Vitro and Ex Vivo Models / Verhoeckx, K., Cotter, Paul, López-Expósito, Iván, Springer International Publishing - ISBN 9783319161044 - p. 255 - 262.
Department(s) Human Nutrition (HNE)
Chair Nutrition and Pharmacology (HNE)
Publication type Peer reviewed book chapter
Publication year 2015
Keyword(s) Ex vivo - Gastro-intestinal hormone release - Porcine ex vivo intestinal segment model

This chapter describes the use of the porcine ex vivo intestinal segment model. This includes the advantages and disadvantages of the segment model and a detailed description of the isolation and culture as well as the applications of the porcine ex vivo intestinal segment model in practice. Compared to the Ussing chamber (Chap. 24) the porcine ex vivo small intestinal segment model is a relatively simple to use intestinal tissue model. The main difference being that the tissue segment is not mounted in a chamber, but is freely floating in a solution. Therefore the ex vivo intestinal segment model does not distinguish between the apical and basolateral side of the tissue. The intestinal segments can be obtained from various anatomical regions of the small intestine (e.g. duodenum, jejunum, ileum or even the colon) and the segments consist of various cell types (e.g. epithelial cells, paneth cells, goblet cells, enterochromaffin cells and enteroendocrine cells). The intestinal segment model has been shown to be a suitable tool to study compound and location specific effects on the release of gastrointestinal hormones and gastrointestinal metabolism of endocannabinoids and related compounds.

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