Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 498095
Title A preliminary amplified fragment length polymorphism (AFLP) primers selection for spathoglottis species
Author(s) Ginibun, F.C.; Bhassu, S.; Khalid, N.; Arens, P.; Vosman, B.; Othman, R.Y.
Source Acta Horticulturae 1078 (2015). - ISSN 0567-7572 - p. 167 - 173.
Event II International Orchid Symposium, Bangkok, Thailand, 2015-03-27
DOI http://dx.doi.org/10.17660/ActaHortic.2015.1078.24
Department(s) WUR PB Siergewassen, Tissue Culture
WUR PB Non host en Insectenresistentie
Publication type Refereed Article in a scientific journal
Publication year 2015
Keyword(s) AFLP primer - Molecular marker - Terrestrial orchid
Abstract

Spathoglottis is a native terrestrial orchid that attracting horticultural interest as a landscaping plant. Distinguishing orchid at species level based on morphological characteristics is unconvincing as some characteristic may sensitive to environmental. Therefore, molecular characterization could be an alternative method in characterizing Spathoglottis species. Amplified Fragment Length Polymorphism (AFLP) marker was selected to characterize the Spathoglottis species. Among 56 AFLP primer combinations tested on Spathoglottis plicata, only eight primer combinations gave clear scorable polymorphic bands. A total of 60 accessions of Spathoglottis species, hybrid and outgroup (Dendrobium) were analysed using the eight primer combinations and generated 891 polymorphic bands with 98.41% in average. The AFLP patterns showed that the Spathoglottis species sampled were clearly different.

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