|Title||A first pedigree-based analysis (PBA) approach for the dissection of disease resistance traits in grapevine hybrids|
|Author(s)||Peressotti, E.; Dolzani, C.; Poles, L.; Banchi, E.; Stefanini, M.; Salamini, F.; Velasco, R.; Vezzulli, S.; Riaz, S.; Walker, M.A.; Reisch, B.I.; De Weg, W.E. Van; Bink, M.C.A.M.|
|Source||Acta Horticulturae 1082 (2015). - ISSN 0567-7572 - p. 113 - 122.|
|Event||XI International Conference on Grapevine Breeding and Genetics, Yanqing, Beijing, China , 2015-04-30/2015-04-30|
WUR PB Biodiversiteit en Genetische Variatie
|Publication type||Refereed Article in a scientific journal|
|Keyword(s)||Downy mildew - Identity by descent - Marker-assisted breeding - Plasmopara viticola - SSR markers - Vitis spp.|
To date, molecular markers are available for many economically important traits in grapevine (Vitis vinifera L.) as well as in other fruit crops. Unfortunately, lack of knowledge of the allelic variation of the related genes hampers their full exploitation in commercial breeding programs. These markers have usually been identified in a single cross. Consequently, only one or two favourable alleles of the related quantitative trait loci (QTL) are identified and exploitable for markerassisted breeding (MAB), whereas a breeding program may include several alleles. Our novel employed approach, namely pedigree-based analysis (PBA), allows for the identification and exploitation of most alleles present in an ongoing breeding program. This is achieved by including breeding material itself in QTL detection, thus covering multiple generations and linking many crosses through their common ancestors in the pedigree. In this study we focus on the genetic characterization of numerous Vitis hybrids, donors of resistance to downy and/or powdery mildew, in order to identify selection signatures. In particular, 120 hybrids of American origin and 100 European hybrids, along with 44 related V. Vinifera cultivars, have been genotyped at 190 SSR loci. These markers have been chosen based on their physical distance in order to have 10 SSRs well-scattered along each chromosome. Based on the SSR profiles, the historical pedigree records have been verified. Following the trueness-to-type validation for most of the studied hybrids, the identity-by-descent (IBD) analysis has been performed, tracing the allelic flow through the successive generations. Here we report results about the identification of non-vinifera genomic intervals retained into the vinifera background and the anchoring of the discovered breeding signatures to the grapevine genome. In addition, we report on the PBAbased QTL results for segregating populations coming from pedigree-supported parental genotypes donors of resistance to downy mildew in north-eastern Italy.