Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 498160
Title Towards identification of the gene responsible for the columnar phenotype of 'McIntosh 'Wijcik" apple
Author(s) Wolters, P.J.; Schouten, H.J.; Velasco, R.; Salvi, S.; Komjanc, M.; Si-Ammour, A.; Baldi, P.
Source In: Acta Horticulturae International Society for Horticultural Science (Acta Horticulturae ) - ISBN 9789462610989 - p. 55 - 62.
Department(s) Laboratory of Plant Breeding
WUR PB Biodiversiteit en Genetische Variatie
Publication type Contribution in proceedings
Publication year 2015
Keyword(s) Bac library screening - Fine mapping - SSR markers - Tree architecture

The 'Wijcik' mutant of the apple cultivar 'McIntosh' shows columnar-type growth, characterized by altered branching and a thick stem. Instead of normal branches, spur-like branches are produced on 'Wijcik' trees, leading to apples produced close to the stem. Columnar-type growth is a desirable trait, as columnar apple trees need considerably less pruning compared to standard trees. Furthermore, apple picking and the application of chemicals is much easier on columnar trees. The genetic basis for columnar growth is a somatic mutation that results in dominant inheritance of the columnar growth trait. The columnar gene (Co) has been previously mapped on apple linkage group 10. Three adult segregating populations, with a total of 301 F1 plants, and two three-year old populations of 1,250 F1 plants were used for fine mapping of the Co region, using newly developed simple sequence repeat (SSR) markers that were designed based on the 'Golden Delicious' apple genome sequence. Co was found to cosegregate with SSR marker Co04R12 and the Co region was found to be delimited by SSR markers Co04R11 and Co04R13 corresponding to a genetic region of 0.56 cM and a genomic region of 393 kb in the 'Golden Delicious' genome. By using BAC libraries, we could identify the clones covering the Co region in 'McIntosh' and 'Wijcik'. This enabled us to sequence the complete Co region in both cultivars. Sequence comparison between 'McIntosh' and 'Wijcik' lead to the identification of a 1,956 bp insertion in 'Wijcik'. This work resulted in the development of a perfect marker for columnar growth for application in breeding, and, in the future, will result in better understanding of the physiological background of columnar growth and the availability of the Co gene for biotechnological applications to manipulate tree architecture.

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