|Title||Selective extraction of intracellular components from the microalga Chlorella vulgaris by combined pulsed electric field-temperature treatment|
|Author(s)||Postma, P.R.; Pataro, G.; Capitoli, M.; Barbosa, M.J.; Wijffels, R.H.; Eppink, M.H.M.; Olivieri, G.; Ferrari, G.|
|Source||Bioresource Technology 203 (2016). - ISSN 0960-8524 - p. 80 - 88.|
|Publication type||Refereed Article in a scientific journal|
|Keyword(s)||Bead mill - Microalgae - Mild cascade biorefinery - Pulsed electric field - Temperature|
The synergistic effect of temperature (25-65°C) and total specific energy input (0.55-1.11kWhkgDW -1) by pulsed electric field (PEF) on the release of intracellular components from the microalgae Chlorella vulgaris was studied. The combination of PEF with temperatures from 25 to 55°C resulted in a conductivity increase of 75% as a result of cell membrane permeabilization. In this range of temperatures, 25-39% carbohydrates and 3-5% proteins release occurred and only for carbohydrate release a synergistic effect was observed at 55°C. Above 55°C spontaneous cell lysis occurred without PEF. Combined PEF-temperature treatment does not sufficiently disintegrate the algal cells to release both carbohydrates and proteins at yields comparable to the benchmark bead milling (40-45% protein, 48-58% carbohydrates).