Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 498382
Title Circulating human CD27-IgA+ memory B cells recognize bacteria with polyreactive Igs
Author(s) Berkowska, Magdalena A.; Schickel, Jean Nicolas; Grosserichter-Wagener, Christina; Ridder, Dick De; Ng, Yen Shing; Dongen, Jacques J.M. Van; Meffre, Eric; Zelm, Menno C. Van
Source The Journal of Immunology 195 (2015)4. - ISSN 0022-1767 - p. 1417 - 1426.
DOI http://dx.doi.org/10.4049/jimmunol.1402708
Department(s) Bioinformatics
EPS
Publication type Refereed Article in a scientific journal
Publication year 2015
Abstract

The vast majority of IgA production occurs in mucosal tissue following T cell-dependent and T cell-independent Ag responses. To study the nature of each of these responses, we analyzed the gene-expression and Ig-reactivity profiles of T cell-dependent CD27+ IgA+ and T cell-independent CD27-IgA+ circulating memory B cells. Gene-expression profiles of IgA+ subsets were highly similar to each other and to IgG+ memory B cell subsets, with typical upregulation of activation markers and downregulation of inhibitory receptors. However, we identified the mucosa-associated CCR9 and RUNX2 genes to be specifically upregulated in CD27-IgA+ B cells.We also found that CD27-IgA+ B cells expressed Abs with distinct Ig repertoire and reactivity compared with those from CD27+IgA+ B cells. Indeed, Abs from CD27-IgA+ B cells were weakly mutated, often used Igl chain, and were enriched in polyreactive clones recognizing various bacterial species. Hence, T cell-independent IgA responses are likely involved in the maintenance of gut homeostasis through the production of polyreactive mutated IgA Abs with cross-reactive anticommensal reactivity.

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