|Title||Transcriptomic profiling of Arabidopsis gene expression in response to varying micronutrient zinc supply|
|Author(s)||Azevedo, Herlânder; Azinheiro, Sarah Gaspar; Muñoz-Mérida, Antonio; Castro, Pedro Humberto; Huettel, Bruno; Aarts, Mark M.G.; Assunção, Ana G.L.|
|Source||Genomics Data 7 (2016). - ISSN 2213-5960 - p. 256 - 258.|
|Department(s)||Groep KoornneefGroep Koornneef
|Publication type||Refereed Article in a scientific journal|
|Keyword(s)||Arabidopsis - BZIP - Microarray - Micronutrient - Zinc deficiency|
Deficiency of the micronutrient zinc is a widespread condition in agricultural soils, causing a negative impact on crop quality and yield. Nevertheless, there is an insufficient knowledge on the regulatory and molecular mechanisms underlying the plant response to inadequate zinc nutrition . This information should contribute to the development of plant-based solutions with improved nutrient-use-efficiency traits in crops. Previously, the transcription factors bZIP19 and bZIP23 were identified as essential regulators of the response to zinc deficiency in Arabidopsis thaliana . A microarray experiment comparing gene expression between roots of wild-type and the mutant bzip19 bzip23, exposed to zinc deficiency, led to the identification of differentially expressed genes related with zinc homeostasis, namely its transport and plant internal translocation . Here, we provide the detailed methodology, bioinformatics analysis and quality controls related to the microarray gene expression profiling published by Assunção and co-workers . Most significantly, the present dataset comprises new experimental variables, including analysis of shoot tissue, and zinc sufficiency and excess supply. Thus, it expands from 8 to 42 microarrays hybridizations, which have been deposited at the Gene Expression Omnibus (GEO) under the accession number GSE77286. Overall, it provides a resource for research on the molecular basis and regulatory events of the plant response to zinc supply, emphasizing the importance of Arabidopsis bZIP19 and bZIP23 transcription factors.