Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 498444
Title Self-seeding microwell chip for the isolation and characterization of single cells
Author(s) Swennenhuis, Joost F.; Tibbe, Arjan G.J.; Stevens, Michiel; Katika, Madhumohan R.; Dalum, Joost Van; Duy Tong, Hien; Rijn, C.J.M. Van; Terstappen, Leon W.M.M.
Source Lab on a Chip 15 (2015)14. - ISSN 1473-0197 - p. 3039 - 3046.
DOI https://doi.org/10.1039/c5lc00304k
Department(s) Laboratory for Organic Chemistry
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2015
Abstract

A self-seeding microwell chip is introduced for the isolation and interrogation of single cells. A cell suspension is transferred to a microwell chip containing 6400 microwells, each microwell with a single 5 μm pore in the bottom. The fluid enters the microwell and drags a cell onto the pore. After a cell has landed onto the pore, it will stop the fluid flow through this microwell. The remaining fluid and cells will be diverted to the next available microwell. This results in a fast and efficient distribution of single cells in individual microwells. After identification by fluorescence microscopy, the cells of interest are isolated from the microwell by punching the bottom together with the cell. The overall single cell recovery of seeding followed by isolation of the single cell, is >70% with a specificity of 100% as confirmed by the genetic make-up of the isolated cells.

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