Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 498615
Title Automated video tracking of thrips behavior to assess host-plant resistance in multiple parallel two-choice setups
Author(s) Thoen, Manus P.M.; Kloth, Karen J.; Wiegers, Gerrie L.; Krips, Olga E.; Noldus, Lucas P.J.J.; Dicke, Marcel; Jongsma, Maarten A.
Source Plant Methods 12 (2016)1. - ISSN 1746-4811 - p. 1 - 12.
DOI http://dx.doi.org/10.1186/s13007-016-0102-1
Department(s) Laboratory of Entomology
PRI Bioint Entomology & Disease Management
PE&RC
EPS
PRI BIOS Applied Metabolic Systems
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) Arabidopsis - Automated video tracking - High-throughput phenotyping - Host-plant resistance - Western flower thrips - 016-3910
Abstract

Background: Piercing-sucking insects cause severe damage in crops. Breeding for host-plant resistance can significantly reduce the yield losses caused by these insects, but host-plant resistance is a complex trait that is difficult to phenotype quickly and reliably. Current phenotyping methods mainly focus on labor-intensive and time-consuming end-point measurements of plant fitness. Characterizing insect behavior as a proxy for host-plant resistance could be a promising time-saving alternative to end-point measurements. Results: We present a phenotyping platform that allows screening for host-plant resistance against Western flower thrips (WFT, Frankliniella occidentalis (Pergande)) in a parallel two-choice setup using automated video tracking of thrips behavior. The platform was used to establish host-plant preference of WFT with a large plant population of 345 wild Arabidopsis accessions and the method was optimized with two extreme accessions from this population that differed in resistance towards WFT. To this end, the behavior of 88 WFT individuals was simultaneously tracked in 88 parallel two-choice arenas during 8 h. Host-plant preference of WFT was established both by the time thrips spent on either accession and various behavioral parameters related to movement (searching) and non-movement (feeding) events. Conclusion: In comparison to 6-day end-point choice assays with whole plants or detached leaves, the automated video-tracking choice assay developed here delivered similar results, but with higher time- and resource efficiency. This method can therefore be a reliable and effective high throughput phenotyping tool to assess host-plant resistance to thrips in large plant populations.

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