|Title||Transient-state kinetic analysis of complex formation between photoprotein clytin and GFP from jellyfish Clytia gregaria|
|Author(s)||Eremeeva, Elena V.; Berkel, Willem J.H. Van; Vysotski, Eugene S.|
|Source||FEBS Letters 590 (2016)3. - ISSN 0014-5793 - p. 307 - 316.|
|Publication type||Refereed Article in a scientific journal|
|Keyword(s)||aequorin - bioluminescence - coelenterazine - FRET - obelin - protein-protein interaction|
Luminous organisms use different protein-mediated strategies to modulate light emission color. Here, we report the transient-state kinetic studies of the interaction between photoprotein clytin from Clytia gregaria and its antenna protein, cgreGFP. We propose that cgreGFP forms a transient complex with Ca2+-bound clytin before the excited singlet state of the coelenteramide product is formed. From the spectral distribution and donor-acceptor separation distance, we infer that clytin reaction intermediates may interact only with the middle side part of cgreGFP.