Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 498666
Title Transient-state kinetic analysis of complex formation between photoprotein clytin and GFP from jellyfish Clytia gregaria
Author(s) Eremeeva, Elena V.; Berkel, Willem J.H. Van; Vysotski, Eugene S.
Source FEBS Letters 590 (2016)3. - ISSN 0014-5793 - p. 307 - 316.
Department(s) Biochemistry
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) aequorin - bioluminescence - coelenterazine - FRET - obelin - protein-protein interaction

Luminous organisms use different protein-mediated strategies to modulate light emission color. Here, we report the transient-state kinetic studies of the interaction between photoprotein clytin from Clytia gregaria and its antenna protein, cgreGFP. We propose that cgreGFP forms a transient complex with Ca2+-bound clytin before the excited singlet state of the coelenteramide product is formed. From the spectral distribution and donor-acceptor separation distance, we infer that clytin reaction intermediates may interact only with the middle side part of cgreGFP.

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