Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 498747
Title Temporal dynamics of the metabolically active rumen bacteria colonizing fresh perennial ryegrass
Author(s) Huws, Sharon A.; Edwards, Joan E.; Creevey, Christopher J.; Stevens, Pauline Rees; Lin, Wanchang; Girdwood, Susan E.; Pachebat, Justin A.; Kingston-Smith, Alison H.
Source FEMS Microbiology Ecology 92 (2016)1. - ISSN 0168-6496 - p. 1 - 12.
DOI http://dx.doi.org/10.1093/femsec/fiv137
Department(s) Microbiological Laboratory
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) 16S - Bacteria - Metagenome - Microbiome - Perennial ryegrass - RNA - Rumen
Abstract

This study investigated successional colonization of fresh perennial ryegrass (PRG) by the rumen microbiota over time. Fresh PRG was incubated in sacco in the rumens of three Holstein × Friesian cows over a period of 8 h, with samples recovered at various times. The diversity of attached bacteria was assessed using 454 pyrosequencing of 16S rRNA (cDNA). Results showed that plant epiphytic communities either decreased to low relative abundances or disappeared following rumen incubation, and that temporal colonization of the PRG by the rumen bacteria was biphasic with primary (1 and 2 h) and secondary (4-8 h) events evident with the transition period being with 2-4 h. A decrease in sequence reads pertaining to Succinivibrio spp. and increases in Pseudobutyrivibrio, Roseburia and Ruminococcus spp. (the latter all order Clostridiales) were evident during secondary colonization. Irrespective of temporal changes, the continually high abundances of Butyrivibrio, Fibrobacter, Olsenella and Prevotella suggest that they play a major role in the degradation of the plant. It is clear that a temporal understanding of the functional roles of these microbiota within the rumen is now required to unravel the role of these bacteria in the ruminal degradation of fresh PRG.

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