Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 498804
Title Bacterial antisense RNAs are mainly the product of transcriptional noise
Author(s) Lloréns-Rico, V.; Cano, J.; Kamminga, T.; Gil, R.; Latorre, A.; Chen, Wei-Hua; Bork, P.; Glass, J.I.; Serrano, L.; Lluch-Senar, M.
Source Science Advances 2 (2016)3. - ISSN 2375-2548
DOI http://dx.doi.org/10.1126/sciadv.1501363
Department(s) Systems and Synthetic Biology
Publication type Refereed Article in a scientific journal
Publication year 2016
Abstract cis-Encoded antisense RNAs (asRNAs) are widespread along bacterial transcriptomes. However, the role of most of these RNAs remains unknown, and there is an ongoing discussion as to what extent these transcripts are the result of transcriptional noise. We show, by comparative transcriptomics of 20 bacterial species and one chloroplast, that the number of asRNAs is exponentially dependent on the genomic AT content and that expression of asRNA at low levels exerts little impact in terms of energy consumption. A transcription model simulating mRNA and asRNA production indicates that the asRNA regulatory effect is only observed above certain expression thresholds, substantially higher than physiological transcript levels. These predictions were verified experimentally by overexpressing nine different asRNAs in Mycoplasma pneumoniae. Our results suggest that most of the antisense transcripts found in bacteria are the consequence of transcriptional noise, arising at spurious promoters throughout the genome
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