Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 498988
Title Purification and immuno-gold labeling of lily mottle virus from lily leaves
Author(s) Zhang, Yubao; Wang, Yajun; Xie, Zhongkui; Wang, Ruoyu; Guo, Zhihong; Werf, Wopke van der; Wang, Le
Source Journal of Virological Methods 232 (2016). - ISSN 0166-0934 - p. 33 - 38.
DOI https://doi.org/10.1016/j.jviromet.2016.02.001
Department(s) Crop and Weed Ecology
PE&RC
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) Immuno-gold labeling assay - Lily mottle virus - Purification - Transmission electron microscope
Abstract

Lily mottle virus (LMoV) is prevalent in Lilium species worldwide causing dwarfing, flower breaking, and reduced bulb yield. In this paper, an easy to use and efficient procedure is described for purification of LMoV from lily leaves. The resulting sample is characterized by a 260/280 nm absorbance ratio of 1.20 at a concentration of 1.27 mg/ml. The procedure results in high protein purity and particle integrity as shown by UV-spectrophotometry, polyacrylamide gel electrophoresis (PAGE), Western blotting, reverse transcriptase (RT)-PCR and transmission electron microscopy (TEM) in combination with immuno-gold labeling. This is the first time that an immuno-gold labeling (IGL) assay was performed to identify a virus of lily. Purified products can be used as a source of antigen in the preparation of antibodies against LMoV and may assist in the development of a diagnostic test for LMoV and in epidemiological surveys.

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