Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 498989
Title Identifying inhibitory effects of lignocellulosic by-products on growth of lactic acid producing micro-organisms using a rapid small-scale screening method
Author(s) Pol, Edwin C. van der; Vaessen, Evelien; Weusthuis, Ruud A.; Eggink, Gerrit
Source Bioresource Technology 209 (2016). - ISSN 0960-8524 - p. 297 - 304.
DOI https://doi.org/10.1016/j.biortech.2016.03.037
Department(s) Bioprocess Engineering
Food Process Engineering
VLAG
FBR Bioconversion
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) Bacteria - Inhibition - Lactic acid - Lignocellulosic by-products - Screening
Abstract

Sugars obtained from pretreated lignocellulose are interesting as substrate for the production of lactic acid in fermentation processes. However, by-products formed during pretreatment of lignocellulose can inhibit microbial growth. In this study, a small-scale rapid screening method was used to identify inhibitory effects of single and combined by-products on growth of lactic acid producing micro-organisms. The small-scale screening was performed in 48-well plates using 5 bacterial species and 12 by-products. Large differences were observed in inhibitory effects of by-products between different species. Predictions can be made for growth behaviour of different micro-organisms on acid pretreated or alkaline pretreated bagasse substrates using data from the small-scale screening. Both individual and combined inhibition effects were shown to be important parameters to predict growth. Synergy between coumaric acid, formic acid and acetic acid is a key inhibitory parameter in alkaline pretreated lignocellulose, while furfural is a key inhibitor in acid pretreated lignocellulose.

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