|Title||Identifying inhibitory effects of lignocellulosic by-products on growth of lactic acid producing micro-organisms using a rapid small-scale screening method|
|Author(s)||Pol, Edwin C. van der; Vaessen, Evelien; Weusthuis, Ruud A.; Eggink, Gerrit|
|Source||Bioresource Technology 209 (2016). - ISSN 0960-8524 - p. 297 - 304.|
Food Process Engineering
|Publication type||Refereed Article in a scientific journal|
|Keyword(s)||Bacteria - Inhibition - Lactic acid - Lignocellulosic by-products - Screening|
Sugars obtained from pretreated lignocellulose are interesting as substrate for the production of lactic acid in fermentation processes. However, by-products formed during pretreatment of lignocellulose can inhibit microbial growth. In this study, a small-scale rapid screening method was used to identify inhibitory effects of single and combined by-products on growth of lactic acid producing micro-organisms. The small-scale screening was performed in 48-well plates using 5 bacterial species and 12 by-products. Large differences were observed in inhibitory effects of by-products between different species. Predictions can be made for growth behaviour of different micro-organisms on acid pretreated or alkaline pretreated bagasse substrates using data from the small-scale screening. Both individual and combined inhibition effects were shown to be important parameters to predict growth. Synergy between coumaric acid, formic acid and acetic acid is a key inhibitory parameter in alkaline pretreated lignocellulose, while furfural is a key inhibitor in acid pretreated lignocellulose.