Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 499517
Title epiGBSBS: reference-free reduced representation bisulfite sequencing
Author(s) Gurp, T. van; Wagemaker, C.A.M.; Wouters, B.; Vergeer, P.; Ouborg, N.J.; Verhoeven, Koen J.F.
Source Nature Methods : techniques for life scientists and chemists 13 (2016). - ISSN 1548-7091 - p. 322 - 324.
DOI https://doi.org/10.1038/nmeth.3763
Department(s) Nature Conservation and Plant Ecology
PE&RC
Publication type Refereed Article in a scientific journal
Publication year 2016
Abstract We describe epiGBSBS, a reduced representation bisulfite sequencing method for cost-effective exploration and comparative analysis of DNADNADNA methylation and genetic variation in hundreds of samples de novo. This method uses genotyping by sequencing of bisulfite-converted DNADNADNA followed by reliable de novo reference construction, mapping, variant calling, and distinction of single-nucleotide polymorphisms (SNSNPs) versus methylation variation (software is available at https://github.com/thomasvangurp/epiGBS). The output can be loaded directly into a genome browser for visualization and into RnBeads for analysis of differential methylation.
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