Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 501686
Title Design, implementation and interpretation of in vitro batch culture experiments to assess enteric methane mitigation in ruminants-a review
Author(s) Yáñez-Ruiz, D.R.; Bannink, A.; Dijkstra, Jan; Kebreab, E.; Morgavi, D.P.; O'Kiely, P.; Reynolds, C.K.; Schwarm, A.; Shingfield, K.J.; Yu, Z.; Hristov, A.N.
Source Animal Feed Science and Technology 216 (2016). - ISSN 0377-8401 - p. 1 - 18.
Department(s) LR - Animal Nutrition
Animal Nutrition
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) Feed evaluation - In vitro gas production - Methane - Microbial inoculum - Mitigation - Rumen

In vitro fermentation techniques (IVFT) have been widely used to evaluate the nutritive value of feeds for ruminants and in the last decade to assess the effect of different nutritional strategies on methane (CH4) production. However, many technical factors may influence the results obtained. The present review has been prepared by the 'Global Network' FACCE-JPI international research consortium to provide a critical evaluation of the main factors that need to be considered when designing, conducting and interpreting IVFT experiments that investigate nutritional strategies to mitigate CH4 emission from ruminants. Given the increasing and wide-scale use of IVFT, there is a need to critically review reports in the literature and establish what criteria are essential to the establishment and implementation of in vitro techniques. Key aspects considered include: i) donor animal species and number of animal used, ii) diet fed to donor animals, iii) collection and processing of rumen fluid as inoculum, iv) choice of substrate and incubation buffer, v) incubation procedures and CH4 measurements, vi) headspace gas composition and vii) comparability of in vitro and in vivo measurements. Based on an evaluation of experimental evidence, a set of technical recommendations are presented to harmonize IVFT for feed evaluation, assessment of rumen function and CH4 production.

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