Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 501732
Title Conversion of polyhydroxyalkanoates to methyl crotonate using whole cells
Author(s) Spekreijse, J.; Holgueras Ortega, J.; Sanders, J.P.M.; Bitter, J.H.; Scott, E.L.
Source Bioresource Technology 211 (2016). - ISSN 0960-8524 - p. 267 - 272.
Department(s) Biobased Chemistry and Technology
Biobased Products
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) Biobased monomer - Downstream processing - Fermentation residues - Methyl crotonate - Polyhydroxyalkanoate

Isolated polyhydroxyalkanoates (PHA) can be used to produce biobased bulk chemicals. However, isolation is complex and costly. To circumvent this, whole cells containing PHA may be used. Here, PHA containing 3-hydroxybutyrate and small amounts of 3-hydroxyvalerate was produced from wastewater and used in the conversion of the 3-hydroxybutyrate monomer to methyl crotonate. Due to the increased complexity of whole cell reaction mixtures compared to pure PHA, the effect of 3-hydroxyvalerate content, magnesium salts and water content was studied in order to evaluate the need for downstream processing. A water content up to 20% and the presence of 3-hydroxyvalerate have no influence on the conversion of the 3-hydroxybutyrate to methyl crotonate. The presence of Mg2+-ions resulted either in an increased yield or in byproduct formation depending on the counter ion. Overall, it is possible to bypass a major part of the downstream processing of PHA for the production of biobased chemicals.

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