|Title||Conversion of polyhydroxyalkanoates to methyl crotonate using whole cells|
|Author(s)||Spekreijse, J.; Holgueras Ortega, J.; Sanders, J.P.M.; Bitter, J.H.; Scott, E.L.|
|Source||Bioresource Technology 211 (2016). - ISSN 0960-8524 - p. 267 - 272.|
Biobased Chemistry and Technology
|Publication type||Refereed Article in a scientific journal|
|Keyword(s)||Biobased monomer - Downstream processing - Fermentation residues - Methyl crotonate - Polyhydroxyalkanoate|
Isolated polyhydroxyalkanoates (PHA) can be used to produce biobased bulk chemicals. However, isolation is complex and costly. To circumvent this, whole cells containing PHA may be used. Here, PHA containing 3-hydroxybutyrate and small amounts of 3-hydroxyvalerate was produced from wastewater and used in the conversion of the 3-hydroxybutyrate monomer to methyl crotonate. Due to the increased complexity of whole cell reaction mixtures compared to pure PHA, the effect of 3-hydroxyvalerate content, magnesium salts and water content was studied in order to evaluate the need for downstream processing. A water content up to 20% and the presence of 3-hydroxyvalerate have no influence on the conversion of the 3-hydroxybutyrate to methyl crotonate. The presence of Mg2+-ions resulted either in an increased yield or in byproduct formation depending on the counter ion. Overall, it is possible to bypass a major part of the downstream processing of PHA for the production of biobased chemicals.