Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 503319
Title Alteration of cell wall polysaccharides through transgenic expression of UDP-Glc 4-epimerase-encoding genes in potato tubers
Author(s) Huang, Jie Hong; Kortstee, Anne; Dees, Dianka C.T.; Trindade, Luisa M.; Schols, Henk A.; Gruppen, Harry
Source Carbohydrate Polymers 146 (2016). - ISSN 0144-8617 - p. 337 - 344.
DOI https://doi.org/10.1016/j.carbpol.2016.03.044
Department(s) Food Chemistry Group
PBR Biobased Economy
WUR PB Biobased Economy
EPS
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) Cell wall polysaccharides - Nucleotide sugar interconversion - Plant biosynthesis - Solanum tuberosum - Transgenic potato - UDP-sugars
Abstract

Uridine diphosphate (UDP)-glucose 4-epimerase (UGE) catalyzes the conversion of UDP-glucose to UDP-galactose. Cell wall materials from the cv. Kardal (wild-type, background) and two UGE transgenic lines (UGE 45-1 and UGE 51-16) were isolated and fractionated. The galactose (Gal) content (mg/100 g tuber) from UGE 45-1 transgenic line was 38% higher than that of wild-type, and resulted in longer pectin side chains. The Gal content present in UGE 51-16 was 17% lower than that of wild-type, although most pectin populations maintained the same level of Gal. Both UGE transgenic lines showed unexpectedly a decrease in acetylation and an increase in methyl-esterification of pectin. Both UGE transgenic lines showed similar proportions of homogalacturonan and rhamnogalacturonan I within pectin backbone as the wild-type, except for the calcium-bound pectin fraction exhibiting relatively less rhamnogalacturonan I. Next to pectin modification, xyloglucan populations from both transgenic lines were altered resulting in different XSGG and XXGG proportion in comparison to wild-type.

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