Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 503453
Title Dietary supplement for energy and reduced appetite containing the β-agonist isopropyloctopamine leads to heart problems and hospitalisations
Author(s) Bovee, Toine F.H.; Mol, Hans G.J.; Bienenmann-Ploum, Monique E.; Heskamp, Henri H.; Bruchem, Gerard D. van; Ginkel, Leendert A. van; Kooijman, Martin; Lasaroms, Johan J.P.; Dam, Ruud van; Hoogenboom, Ron L.A.P.
Source Food Additives & Contaminants. Pt. A, Chemistry, Analysis, Control, Exposure & Risk Assessment 33 (2016)5. - ISSN 1944-0049 - p. 749 - 759.
DOI https://doi.org/10.1080/19440049.2016.1167965
Department(s) RIKILT - BU Toxicology Bioassays & Novel Foods
RIKILT - Business unit Contaminants & Toxins
Rikilt B&T Toxicologie en Effectanalyse
RIKILT Directie
RIKILT - Business unit Dierbehandelingsmiddelen
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) Biosensor - enforcement - health risks - internet - supplements - web shops
Abstract

In 2013 the Dutch authorities issued a warning against a dietary supplement that was linked to 11 reported adverse reactions, including heart problems and in one case even a cardiac arrest. In the UK a 20-year-old woman, said to have overdosed on this supplement, died. Since according to the label the product was a herbal mixture, initial LC-MS/MS analysis focused on the detection of plant toxins. Yohimbe alkaloids, which are not allowed to be present in herbal preparations according to Dutch legislation, were found at relatively high levels (400–900 mg kg–1). However, their presence did not explain the adverse health effects reported. Based on these effects the supplement was screened for the presence of a β-agonist, using three different biosensor assays, i.e. the validated competitive radioligand β2-adrenergic receptor binding assay, a validated β-agonists ELISA and a newly developed multiplex microsphere (bead)-based β-agonist assay with imaging detection (MAGPIX®). The high responses obtained in these three biosensors suggested strongly the presence of a β-agonist. Inspection of the label indicated the presence of N-isopropyloctopamine. A pure standard of this compound was bought and shown to have a strong activity in the three biosensor assays. Analysis by LC-full-scan high-resolution MS confirmed the presence of this ‘unknown known’ β3-agonist N-isopropyloctopamine, reported to lead to heart problems at high doses. A confirmatory quantitative analysis revealed that one dose of the preparation resulted in an intake of 40–60 mg, which is within the therapeutic range of this compound. The case shows the strength of combining bioassays with chemical analytical techniques for identification of illegal pharmacologically active substances in food supplements.

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