Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 503457
Title Application of whole genome shotgun sequencing for detection and characterization of genetically modified organisms and derived products
Author(s) Holst-Jensen, Arne; Spilsberg, Bjørn; Arulandhu, Alfred J.; Kok, Esther; Shi, Jianxin; Zel, Jana
Source Analytical and Bioanalytical Chemistry 408 (2016)17. - ISSN 1618-2642 - p. 4595 - 4614.
DOI http://dx.doi.org/10.1007/s00216-016-9549-1
Department(s) RIKILT - BU Toxicology Bioassays & Novel Foods
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) Cisgene - Intragene - Traceability - Transcriptome sequencing - Transgene - Unknown GMO
Abstract

The emergence of high-throughput, massive or next-generation sequencing technologies has created a completely new foundation for molecular analyses. Various selective enrichment processes are commonly applied to facilitate detection of predefined (known) targets. Such approaches, however, inevitably introduce a bias and are prone to miss unknown targets. Here we review the application of high-throughput sequencing technologies and the preparation of fit-for-purpose whole genome shotgun sequencing libraries for the detection and characterization of genetically modified and derived products. The potential impact of these new sequencing technologies for the characterization, breeding selection, risk assessment, and traceability of genetically modified organisms and genetically modified products is yet to be fully acknowledged. The published literature is reviewed, and the prospects for future developments and use of the new sequencing technologies for these purposes are discussed.

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