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Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 503487
Title Cell diameter doesn't affect lipid productivity of Chlorococcum littorale
Author(s) Dominguez Teles, I.; Gomes Sanches Fernandes, Carolina; Kleinegris, Dorinde M.M.; Wijffels, René H.; Barbosa, Maria J.
Source Algal Research 19 (2016). - ISSN 2211-9264 - p. 333 - 341.
Department(s) Bioprocess Engineering
FBR Bioconversion
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) Biomass productivity - Cell diameter - Cell sorting (FACS) - Chlorococcum littorale - Lipid productivity - Microalgae

We hypothesized that cells with different diameter have different division rates, which could affect lipid productivity (lipid content. ×. biomass productivity). In the present work we assessed the influence of cell diameter, as a sorting parameter, on both biomass and lipid productivity of Chlorococcum littorale. Prior to sorting, cells were grown in a batch-wise nitrogen run-out including a long nitrogen depleted phase (N. -) to stop cell division, thus only having vegetative cells (Pre-sorting). Cell sorting was done at the end of this N. - phase using FACS (fluorescence assisted cell sorting) based on forward scatter as a proxy for diameter (size ranges (μm): 5-6 (small), 8-9 (medium), 11-14 (large) and 5-14 (control)). The sorting was done in 2 pools: multiple-cell (100 cells) and single-cell. After sorting, cells were recovered under low-light for 2. weeks, and used to start the Post-sorting experiment (analogous to Pre-sorting). The populations derived from different sorted pools, single-cell and multiple-cell, showed similar size distributions after re-growth. No difference was observed in biomass and lipid productivities among Post-sorting cells and when compared to Pre-sorting cells under nitrogen depletion. We concluded that cellular size had no effect on both biomass and lipid productivity of C. littorale.

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