Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 503507
Title Influence of nitrogen depletion in the growth of N. oleoabundans on the release of cellular components after beadmilling
Author(s) Günerken, Emre; Hondt, Els D'; Eppink, Michel; Elst, Kathy; Wijffels, Rene
Source Bioresource Technology 214 (2016). - ISSN 0960-8524 - p. 89 - 95.
DOI http://dx.doi.org/10.1016/j.biortech.2016.04.072
Department(s) Bioprocess Engineering
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) Biochemical release - Cell disruption - Microalgae - Nitrogen depletion
Abstract

Cell disruption by bead milling of Neochloris oleoabundans grown under nitrogen repleted (NR) and nitrogen depleted (ND) conditions was evaluated based on the release of biochemicals and biomass to the supernatant. Additionally, energy consumption for cell disruption was calculated per kg of released component. Bead milling of NR cells resulted on average in 34% (w/w) release of biochemicals into the supernatant, with a similar composition as the untreated microalgal biomass. With ND cells, the release was higher and more selective, i.e. 57%, 59%, 68% and 56% (w/w) of respectively biomass, proteins, carbohydrates and lipids whereof 92%, 57% and 46% (w/w) respectively of phospho-, glyco- and neutral lipids.

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