|Title||A new formula to calculate activity of superoxide dismutase in indirect assays|
|Author(s)||Zhang, Chen; Bruins, Marieke E.; Yang, Zhi Qiang; Liu, Shu Tao; Rao, Ping Fan|
|Source||Analytical Biochemistry 503 (2016). - ISSN 0003-2697 - p. 65 - 67.|
Biobased Chemistry and Technology
FBR Food Technology
|Publication type||Refereed Article in a scientific journal|
|Keyword(s)||Cytochrome c - Epinephrine - Hydroxylamine hydrochloride - Nitroblue tetrazolium - SOD - WST|
To calculate superoxide dismutase (SOD) activity rapidly and accurately by indirect SOD assays, a formula based on the ratio of the catalytic speed of SOD to the reaction speed of the indicator with superoxide anion was deduced. The accuracy of this formula was compared with the conventional formula based on inhibition in five indirect SOD assays. The new formula was validated in nearly the entire SOD activity range, whereas the conventional formula was validated only during inhibition of 40-60%. This formula might also be used for the assays of other enzymes.