Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 503538
Title A new formula to calculate activity of superoxide dismutase in indirect assays
Author(s) Zhang, Chen; Bruins, Marieke E.; Yang, Zhi Qiang; Liu, Shu Tao; Rao, Ping Fan
Source Analytical Biochemistry 503 (2016). - ISSN 0003-2697 - p. 65 - 67.
DOI http://dx.doi.org/10.1016/j.ab.2016.03.014
Department(s) Biobased Chemistry and Technology
FBR Food Technology
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) Cytochrome c - Epinephrine - Hydroxylamine hydrochloride - Nitroblue tetrazolium - SOD - WST
Abstract

To calculate superoxide dismutase (SOD) activity rapidly and accurately by indirect SOD assays, a formula based on the ratio of the catalytic speed of SOD to the reaction speed of the indicator with superoxide anion was deduced. The accuracy of this formula was compared with the conventional formula based on inhibition in five indirect SOD assays. The new formula was validated in nearly the entire SOD activity range, whereas the conventional formula was validated only during inhibition of 40-60%. This formula might also be used for the assays of other enzymes.

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